Figure 1.

Tetramer staining of TARP-TCR+ CTLs from pedAML patients. The lymphocyte gating strategy, described in Supplemental Materials, is illustrated in (A). The total lymphocyte compartment is indicated in green, other white blood cells in blue en non-viable cells or doublets in dark grey. Subsequent patient-individual gating of the CD3+/CD8+ compartment, and tetramer staining within this compartment, is shown for three TARP-high/HLA-A∗0201 positive patients in (B-D). Tetramer-positive events, defining TARP-TCR+ CTLs, are indicated in black, and tetramer-negative CTLs in light grey. Non-CD3+/CD8+ cells within the lymphocyte gate are indicated in green. Median MFI values are indicated by circles. Tetramer positivity was gated for each patient individually based on sample-specific FMO controls. For two out of the three patients (B-C), both BM and PB were analysed. For one of the three patients (B), lymphocytes from both diagnosis and relapse could be evaluated. BM = bone marrow, CTL = cytotoxic T-cell, FMO = fluorescence-minus-one, PB = peripheral blood, TARP = T-cell receptor γ chain alternate reading frame protein, TCR = T-cell receptor.