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. 2006 Oct 18;9(4):875–890. doi: 10.1111/j.1462-5822.2006.00837.x

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Association of dsRNA, C and mitochondrial protein P32 with CPVs in replicon‐transfected cells by immuno‐EM (BHK cells transfected with RUBrep/C‐GFP/neo after drug selection). A. Immunogold labelling with anti‐dsRNA antibodies. dsRNA signal associated with a straight element (star) is indicated with an arrow. B–D. Immunogold labelling with anti‐GFP antibodies to detect C‐GFP. Labelling around vacuoles (asterisks) and straight elements (stars) within CPVs is indicated by arrows. In C, exterior labelling of a mitochondrion (mi) is indicated by an arrowhead. E. Immunogold labelling with anti‐P32 antibodies. Labelling of the interior of a CPV is shown with arrows in both the main field and inset (straight elements are indicated by a star). Note the labelling of a neighbouring mitochondrion (mi). Bars: 200 nm.