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. 2008 Jun 10;10(10):2012–2028. doi: 10.1111/j.1462-5822.2008.01184.x

Figure 6.

Figure 6

Tube structure and virus assembly in the presence of drugs that affect Golgi membranes or actin integrity. Immunofluorescence microscopy of GalT and giantin in normally infected cells (5 PFU/cell) at 10 h p.i. (A and B), in infected cells treated 15 min with BFA (C and D) and in infected cells treated 30 min with BFA (E and F).
G and H. EM shows normal tubes out of Golgi stacks after 15 min with BFA.
G. Long tube (arrow) globular domains (arrowheads) and tube attached to mitochondria (double arrow).
H. Cytoplasmic filaments (arrows) apparently attached to a viral tube.
I. Normal tube with cylindrical and globular (arrow) domains after 30 min with BFA.
J and K. Fluorescence microscopy detection of actin (Phalloidin, green) in normally infected cells at 10 h p.i. (J) and in infected cells treated with JPK (drug was added at 1 h p.i.) (K).
L and M. EM of infected cells treated with JPK. Globular domain (arrow in L) in an altered Golgi stack (G) and budding profiles (arrows in M) in plasma membrane. Bars: 200 nm in G, L, M; 100 nm in H and I.