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. 2006 Mar 3;8(7):1147–1157. doi: 10.1111/j.1462-5822.2006.00698.x

Figure 7.

Figure 7

A. Confocal analysis of cells expressing EGFP‐IBV N protein (green) showing low levels of protein in the nucleus but not nucleolus, which has been stained with PI (red), at 24 h post transfection. Separate channels are shown as well as the merged image. The right‐hand images are enhanced magnification images of sections of the nuclear/cytoplasmic boundary showing the nucleoplasm (Np), nucleolus (No) and nuclear envelope (NE).
B. Upper images are examples of differential stained nucleoli in cells expressing EGFP‐IBV N protein (green) and PI to stain the nucleolus (red), and merged. The lower image shows the apparent circumferences of the nucleoli when visualized using EGFP and PI.
C. Orthogonal reconstruction through the cell in (B) by Z‐sectioning. The orthogonal section corresponds to the vertical bar in the lower left‐hand cell shown in (B).
D. Z‐sectioning through the nucleus/nucleolus in a cell expressing ECFP‐NR1+2 and DsRed‐B23 at 24 h post transfection. Also shown is the apparent maximum diameter (indicated) of the FC using the nucleolus shown in the 2.92 µm section. The nucleolus, nucleoplasm and nuclear envelope are indicated No, NP and NE respectively. The horizontal size bar is 10 µm.