TABLE 1.
Nucleotide sequences of the primers and probes used in this study
| Primer or probe | Sequencea |
|---|---|
| hMPVb | |
| Forward primer | 5′-CATATAAGCATGCTATATTAAAAGAGTCTCA-3′ (35) |
| Reverse primer | 5′-CCTATTTCTGCAGCATATTTGTAATCAG-3′ (197) |
| Probe | 5′-FAM-CAACHGCAGTGACACCCTCATCATTGCA-TAMRA-3′ (95) |
| hRSVc | |
| Forward primer | 5′-GCTCTTAGCAAAGTCAAGTTRAATGATACA-3′ (15) |
| Reverse primer | 5′-GTTTTTGCACATCATAATTRGGAGT-3′ (138) |
| Probe | 5′-VIC-CTGTCATCYAGCAAATACACTATCCAACGTAGCACAGG-TAMRA-3′ (63) |
hMPV, human metapneumovirus; hRSV, human respiratory syncytial virus.
H, mix of T, C and A; R, mix of A and G; Y, mix of C and T.
a
The location of the primers and probes is indicated in parentheses (the reference sequence for hMPV is AY145286, and that for hRSV is X00001).
b
Maertzdorf et al. [19], adapted according to the recent sequence information available in GenBank.
c
Designed as part of the present study.