TABLE 2.

Cut-off positive cycle threshold (Ct) values obtained for the analysis of the human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) constructs in single and multiplex real-time PCRs

	Ct single PCR	Ct multiplex PCR	Delta Cta
Concentration hRSV
1010 copies/mL	16.29	16.39	0.10
109 copies/mL	20.74	21.22	0.48
108 copies/mL	25.68	24.49	−1.19
107 copies/mL	27.62	27.86	0.24
106 copies/mL	31.25	31.61	0.36
105 copies/mL	34.52	35.45	0.93
104 copies/mL	39.85	40.20	0.35
R2	0.9958	0.9968
Efficiency (%)	84.20	82.60
Concentration hMPV
1010 copies/mL	16.02	16.56	0.54
109 copies/mL	18.98	19.82	0.84
108 copies/mL	22.46	22.39	−0.07
107 copies/mL	25.87	26.28	0.41
106 copies/mL	29.57	30.02	0.45
105 copies/mL	32.88	33.00	0.12
104 copies/mL	35.83	35.90	0.07
R2	0.9992	0.9978
Efficiency (%)	98.00	101.50

^aDelta Ct = Ct multiplex PCR minus Ct single PCR.