Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Flowchart of a general expression protocol used by the authors to express a broad range of targets, from phosphatases, to neuronal scaffolding proteins, to bacterial signaling proteins. The approximate time required to complete each segment of the protocol is listed to the left of the corresponding step.
