Figure 3.

Influenza virus genomic RNA is not the target of miR‐30. (a) Predicted interactions between miR‐30b/c and the influenza virus M gene using RNA22 V2. (b) Effects of miR‐30a/b/c/d/e mimics on the expression of the firefly luciferase gene from the pmirGLO reporter constructs containing the putative miR‐30b/c binding sites from the M gene (pmirGLO‐M) and 3′UTR of human p53 (pmirGLO‐p53). First, 293T cells were co‐transfected with reporter constructs together with miR‐30a/b/c/d/e mimics or NC. After 24 hr, cells were lysed, and luciferase activities were measured. The luciferase activity was normalised to the Renilla luciferase activity, and the data are expressed relative to that of the NC. The values are shown as the mean and SD and are representative of three independent experiments. Data were analysed using Student's t test. ***p < .001; **p < .01; *p < .05