Figure 1.

Kinetics and rate of DENV internalization into Vero cells.
A. Vero cells were infected with DENV‐2 during 1 h at 4°C and then transferred to 37°C. At the indicated times after incubation at 37°C, extracellular virus was inactivated with proteinase K. Results are shown as a percentage of internalized virus when compared with controls in which PBS was substituted for proteinase K.
B. Vero cells were infected with DENV‐2 at 37°C (non‐synchronized infection) or at 4°C and then transferred to 37°C (synchronized infection). At the indicated times extracellular virus was inactivated with proteinase K. Results are expressed as number of internalized PFU.