Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Jun 11;11(10):1533–1549. doi: 10.1111/j.1462-5822.2009.01345.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Blackwell Publishing Ltd

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

PMC Copyright notice

DENV‐1 and DENV‐2 entry into Vero cells is dependent of acidic pH. A. Cells were treated with ammonium chloride or concanamycin A and infected with DENV‐1 or DENV‐2. After 1 h internalization in the presence of the drugs, extracellular virus was inactivated with proteinase K and the cell pellets were plated onto Vero cells to determine internalized virus by an infectious centre assay. Results are expressed as percentage of internalized virus with respect to a control without drug treatment. Each point shows the mean ± SD of two independent experiments. B. Cells were treated with 50 mM ammonium chloride before or after infection with DENV‐2 or were left untreated (control). At 48 h p.i. immunostaining was carried out using mouse anti‐E glycoprotein antibody and FITC‐labelled anti‐mouse IgG.