Figure 3.

Indomethacin inhibits viral protein synthesis.

A. Mock‐infected (–VSV) or VSV‐infected (+VSV) MA104 cells treated with 400 μM INDO after the 1 h adsorption period were labelled with [³⁵S]‐methionine (4 h pulse) at 4 h p.i. Samples containing equal amounts of proteins (right panel) or equal amounts of radioactivity (left panel) were processed for SDS‐PAGE and autoradiography. VSV proteins L, G, N, NS and M are indicated.

B. Mock‐infected (mock) or VSV‐infected (VSV) MA104 cells treated as in A were labelled with [³⁵S]‐methionine (1 h pulse) at different times p.i. Protein synthesis, determined by [³⁵ S]‐methionine incorporation into proteins of untreated (empty symbols) or INDO‐treated (filled symbols) cells, is expressed as percent of untreated, mock‐infected control.

C. In a parallel experiment, samples containing equal amounts of radioactivity collected at early (1–4 h) and late (5–8 h) times p.i. were processed for SDS‐PAGE and autoradiography (right panel). VSV proteins are indicated as in A. Control cells labelled at 1 and 5 h after mock infection are shown. Levels of viral proteins G, N, NS and M in untreated (empty symbols) or INDO‐treated (filled symbols) samples were determined by densitometric analysis and expressed as arbitrary units (left panel). Data represent results from one experiment representative of three independent experiments with similar results.