Table 1.
Antimetastatic, antitumoral, antiviral, mitogenic and biocide activities of tarin purified by distinct chromatographic procedures
| Bioactivity | Tarin purification steps | Type of assay | Target | Effects | Reference |
|---|---|---|---|---|---|
| Antimetastatic | Ultrafiltration, Size exclusion chromatography (SEC), Anion exchange chromatography | in vivo | Murine mammary tumor cell line 66.1 | Inhibition of lung colonizing ability of 66.1 cells | Kundu et al., 2012 |
| Antitumoral | Ultrafiltration, Anion exchange chromatography, SEC and second anion exchange chromatography | in vitro | Murine mammary tumor cell line 66.1 | 35% inhibition of 66.1 cells proliferation | Kundu et al., 2012 |
| Anion exchange chromatography, Anion exchange and Gel Filtration | Hepatoma HepG2 cells | 22.3 μM tarin reduced 75% of cell proliferation in 48h | Chan et al., 2010 | ||
| Antiviral | Affinity chromatography | in vitro | SARS‐CoV | EC50>60 μg/mL | Keyaerts et al., 2007 |
| FIPV | EC50 >2.5 ± 0.6 μg/mL | ||||
| Mitogenic/ Potential immunomodulator | Anion exchange, Anion exchange and Gel filtration | in vitro | Total spleen cells | Cytokines expression IL‐2, IL‐1β, INF‐γ and TNF‐α | Chan et al., 2010 |
| Affinity chromatography | in vitro | Mice spleen cells mice bone marrow cells | Dose‐dependent proliferative response | Pereira et al., 2014 | |
| Anion exchange, Anion exchange and Gel Filtration | Chan et al., 2010 | ||||
| Affinity chromatography | in vivo | Mice spleen cells | 3.3‐fold proliferation increase on 5th day | Pereira et al., 2014 | |
| Mice spleen B lymphocytes | 4.1‐fold proliferation increase on 5th day | ||||
| Biocide | Affinity chromatography | in vitro | Lipaphis erysimi | LC50 = 11.87 μg/mL | Roy et al., 2014 |
| Gel filtration | Bemisia tabaci | LC50 = 5.17 μg/mL | |||
| Affinity chromatography | in vitro | Dysdercus koenigii/cingulatus | LC50 = 19.9 ± 0.98 μg/mL | Roy et al., 2002 | |
| Tarin 1 expressed in Nicotiana tabacum | in vitro | Spodoptera frugiperda larvae | Provoke higher mortality | Leal‐Bertioli et al., 2003 | |
| Pseudomonas syringae pv. tomato | Growth inhibition | ||||
| Affinity chromatography | in vitro | Bactrocera cucurbitae | LC50 = 51.6 μg/mL | Thakur et al., 2013 | |
| Affinity chromatography and anion exchange chromatography | in vitro | Aphis gossypii | LC50 = 9.98 ± 0.239 μg/mL | ||
| Dysdercus cingulatus | LC50 = 16.95 ± 0.279 μg/mL | ||||
| Aphis craccivora | LC50 = 15.21 ± 0.274 μg/mL |
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