Table 2.
Real‐time RT‐PCR analyses of 15 selected genes expressed in RD cells at 8 and 20 h following infection with EV71 strains MS/7423/87 (MS) and 5865/Sin/000009 (SIN).
| Gene | Fold change | ||||||
|---|---|---|---|---|---|---|---|
| Microarrayanalysis | RT‐PCR 8 h p.i. | RT‐PCR 20 h p.i. | |||||
| Mock | EV71 (MS) | EV71 (SIN) | Mock | EV71 (MS) | EV71 (SIN) | ||
| CDK6 | −3.05 | −1.02 | +1.37 | +1.80 | 1.00 | −2.94 (0.013) | −3.43 (0.031) |
| eEF1G | −2.62 | +1.08 | +1.50 (0.034) | +1.80 (0.023) | −1.08 | −2.85 (0.039) | −3.56 (0.025) |
| IL12A | −3.84 | +1.23 | −1.01 | +1.28 | −1.14 | −2.60 (0.009) | −3.15 (0.018) |
| NPC2 | −2.31 | −1.08 | +1.24 | +3.19 (0.013) | +1.04 | −2.58 (0.004) | −3.07 (0.002) |
| ODC1 | −2.53 | +1.08 | +1.11 | +1.25 | +1.10 | −2.99 (0.007) | −2.35 (0.017) |
| PIM2 | −2.60 | +1.10 | +1.11 | +1.30 | +1.20 | −2.69 (0.027) | −3.17 (0.008) |
| PSMA2 | −3.42 | +1.04 | +1.33 | +1.27 | +1.17 | −4.61 (0.001) | −4.71 (0.001) |
| TPT1 | −3.51 | −1.05 | +1.09 | +1.78 (0.036) | +1.27 | −3.16 (0.008) | −3.07 (0.006) |
| ACTA2 | +2.20 | 1.00 | +4.07 (0.020) | +5.05 (0.006) | +1.21 | +7.75 (0.001) | +8.25 (0.011) |
| AP1S1 | +3.57 | −1.23 | −1.12 | −1.16 | +1.03 | +3.59 (0.030) | +3.31 (0.022) |
| APOB | +5.18 | +1.02 | −1.02 | −1.03 | −1.02 | +8.97 (0.005) | +6.57 (0.007) |
| CHRM2 | +2.51 | +1.04 | +3.11 (0.028) | +3.05 (0.004) | −1.23 | +2.27 (0.033) | +2.14 (0.037) |
| CLU | +2.08 | −1.25 | −1.45 | −1.32 | −1.11 | +3.59 (0.015) | +3.31 (0.030) |
| DCAMKL1 | +14.81 | +1.12 | +1.20 | +1.35 | +1.09 | +35.88 (0.013) | +36.25 (0.033) |
| GTF2F1 | +2.45 | +1.03 | −1.08 | +1.41 | +1.04 | +2.73 (0.004) | +2.80 (0.004) |
Samples were normalized with GAPDH as the control housekeeping gene, and with uninfected RD cells at each respective time‐point as the reference sample (value of 1). The P‐values denoting significant differences (P < 0.05) are shown within parentheses.