Table 3.
Sequences of primers for real‐time RT‐PCR amplification of selected genes.
| Gene | Primer sequence (5′→3′) | Target size(bp) | |
|---|---|---|---|
| Sense | Anti‐sense | ||
| ACTA2 | ATTGACATCAGGAAGGACCT | TGTGTGCTAGAGACAGAGAG | 305 |
| AP1S1 | TGCCAGCCTCTACTTCTGCT | CGATGGCTTTCAGCACACTC | 221 |
| APOB | GCTTCAGCCATTGACATGAG | CTTTGTGTTCAAGAGCTGCA | 277 |
| CDK6 | AAGAATGTTGGCAGGTGACT | TTCAAGAGGGCTTTCTGTGT | 258 |
| CHRM2 | AACCCAGGATGAAAACACAG | GTGATGATGAAAGCCAACAG | 294 |
| CLU | ATTCTGACCAAGGTGCGACA | TGGACAGGAAATGCCACAGT | 265 |
| DCAMKL1 | GCCTGAGTTTCTTCTGGAAC | TACTGTTCAGCCACCAGATG | 209 |
| eEF1G | TCAGACCTTCATGAGCTGCA | TACTCTCGAACCAGCGTCTG | 249 |
| EV71VP1 | TTCAGTAGGGCAGGCTTGGT | GTCTGCCAAGCGAGTGATTC | 269 |
| GAPDH a | AGGTGAAGGTCGGAGTCAAC | CATGGGTGGAATCATATTGG | 153 |
| GTF2F1 | GACCACTAAGGACCTGCTGA | AGTCAGGGAGCAAGCAGGAT | 303 |
| IL12A | CCAGGTGGAGTTCAAGAC | ACAACGGTTTGGAGGGAC | 285 |
| NPC2 | AGCTCTGCTGCTTCAACAAC | AGGTGTAGAAAGAGGCCACA | 223 |
| ODC1 | ATACTCTATGACCACGCACA | GCCCTGACATCACATAGTAG | 250 |
| PIM2 | GGAGATTCTGGAAGCTGAG | TGTCCATCTATCCCTGTGA | 286 |
| PSMA2 | AAGGGCAAATGACAGAGGAT | TGGAAGGTGGGTTTAACAGT | 276 |
| TPT1 | ACCGAAAGCACAGTAATCAC | CACGGTAGTCCAATAGAGCA | 274 |
a
. Housekeeping gene serving as the normalization control.