Figure 4.

Effect of the inhibition of GP‐cleavage on the fusion activity of surface‐expressed BDV‐GP.

A. Confluent Vero E6 cell cultures either BDV‐infected or non‐infected as control were incubated at a pH of either 7.0 or 5.5 for 5 min, incubated for 90 min in normal culture medium and fixed and stained with Giemsa.

B. BDV‐infected Vero E6 cells were incubated with the indicated concentrations of MI‐0701 and grown to confluence. The cells were then incubated at a pH of 5.5 as described in (A), fixed and stained with Giemsa.

C. Quantification of two independent experiments, conducted as described in (B). For quantification, the number of nuclei within syncytia was divided by the total number of nuclei.