Table 2.

Primer sequences for real‐time PCR

Gene of interest	Primer sequence (5′ to 3′)
RIG‐I	Forward primer: GGCAAGTCCCGCTGTAAAC Reverse Primer: TTGGTATCTCCTAATCGCAAAAG
MDA‐5	Forward primer: TGGACAAGCTTCTAGTTAGAGACG Reverse Primer: GATTCATTTCCATTGTTTTCTGC
IFN‐α	Forward primer: CCATAGCCTGGATAACCGTCG Reverse Primer: TCTTGGGGAAAGCCAAAGTCA
IL‐1β	Forward primer: TACAGTGGCAATGAGGAT Reverse Primer: ATGAAGGGAAAGAAGGTG
IL‐8	Forward primer: ATGACTTCCAAGCTGGCCGTGGCT Reverse Primer: TCTCA GCCCTCTTCAAAAACTTCTC
GAPDH	Forward primer: GAAGGTGAAGGTCGGAGTC Reverse Primer: GAAGATGGTGATGGGATTTC

IFN‐α, interferon alpha; IL, interleukin; GADPH, glyceraldehyde‐3‐phosphate dehydrogenase; MDA‐5, melanoma differentiation associated gene 5; RIG‐I, retinoic acid inducible gene I.