Table 3.

Estimated Quantity of Foreign Protein Produced by a Given Production Systema

System	Vol. of medium in system	Total cells in system (vol. × conc.)	Total product in systemb
Batch T‐flask (T‐175):
175 cm2 attached growth in DMEM/F12/10% serumc	75 ml	1.8 × 107	0.5‐1 mg
Batch shake flask (250 ml), 0.5‐1% serumc	100 ml	2 × 108	2‐4 mg
Batch 5‐liter spinner flaskc	1000 ml	1 × 109	10‐20 mg
Batch 5‐liter spinner flask fitted with O2 frit spargerc	1000 ml	2 × 109	20‐40 mg
Batch 15‐liter bioreactor with pH and O2 controlc	10 liters	2 × 1010	200‐400 mg
Continuous‐mode 15‐liter perfusion reactor with pH and O2 control and cell retention	5‐20 liter perfusion per day	1 × 1011	100‐400 mg per day

^aQuantities assume a specific productivity of 1 to 4 mg/10⁹ viable cells per day.

^bProduction of recombinant intracellular protein may achieve ∼0.1% of total cell protein.

^cBatch culture would be performed for 4 days after inoculation of 1 × 10⁵ viable cells/ml.

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