Table 4.

Typical Protein Requirements for Various Analyses

Assay	Quantity of protein required	Comments
ELISA	<5 µg	Identification of activity possible with crude extract
Amino acid analysis and sequencing (including development of method)	20 µg	Pure protein required thus starting requirement would be >100 µg in order to account for purification losses
Carbohydrate analysis	20 µg	Pure protein required
Extinction‐coefficient determination	500 µg	Pure protein required; thus starting requirement would be 3‐5 mg
Biological testing in animal‐based model	50 mg	Must obtain suitable purity of product to prevent collateral effects on physiology due to contaminants
Human clinical trials	1‐100 g	>95% purity requires extensive testing to ensure safety

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