Figure 3.

BLI of MHV‐EFLM and MHV‐2aFLS: spatial and temporal progression of infection. The 6‐ to 8‐week‐old female BALB/c mice were inoculated intranasally with 10⁶ TCID₅₀ of either MHV‐EFLM or MHV‐2aFLS. At the indicated times post inoculation (days p.i.), mice were processed for BLI as described in the Experimental procedures.
A. Dorsal and ventral images of a representative mouse, infected either with MHV‐EFLM or with MHV‐2aFLS, are shown. The emitted photons were measured by a CCD camera and are displayed as a heat map in an overlay image. Note that the scaling [in arbitrary units (AU)] differs between the dorsal and ventral images.
B. The total amounts of emitted photons from the dorsal head regions at the different time points were quantified and are expressed as integrated intensities on a log² scale with standard deviations (n = 4).