Figure 7.

EWI‐2wint increases CD81–CLDN1 colocalization.

A. Representative images from confocal microscopy experiments. Fixed Huh‐7 cells expressing pcDNA3.1 (left) EWI‐2 (middle) or EWI‐2wint 3 (right) were labelled with TS81 anti‐CD81 and anti‐CLDN1 mAbs followed by secondary antibodies conjugated with Alexa488 (green staining) and Alexa555 (red staining) respectively.

B. Colocalization was calculated as the number of yellow pixels (green and red pixels) over the number of yellow + red pixels, using CoLocalizer Pro software. *** means a P value below 0.001 as determined by the Mann–Whitney U ‐test.