Figure 2.

Ultrastructural analysis of E.Derm cells infected with BEV processed by cryosubstitution or cryosectioning.

A–D. Electron micrographs of E.Derm cells infected with BEV for 16 h that were subjected to (A, B) freeze substitution before embedding them in Lowicryl HM20 resin or (C, D) cryosectioning as described in the Experimental Procedures section. Clusters of DMVs (white asterisks) surrounded by mitochondria and ER were observed in the infected cells. Viral particles could be visualized outside the clusters (white arrows). The boxed area in A is enlarged in B. M: Mitochondria; ER: Endoplasmic reticulum; N: nucleus. Scale bars, 200 nm.