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. 2016 Jul 5;18(12):1691–1708. doi: 10.1111/cmi.12620

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© 2016 John Wiley & Sons Ltd

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Relationship between the DMVs and the RTCs in BEV‐infected cells.

A. Cryosections of E.Derm cells infected with BEV and fixed at 16 h pi were immunogold labelled with a mAb anti‐dsRNA (left panel) or with antibodies against the RdRp (middle panel) and M^pro (right panel) and analysed by electron microscopy. Scale bars, 100 nm.

B. Immunodetection of M^pro and RdRp in a membrane‐enriched cell fraction analysed by Western blot. Mock‐infected and BEV‐infected (16 h) E.Derm cells were fractionated by differential centrifugation into cytosolic (S100) and membranous (P100) fractions and analysed by Western blot with anti‐M^pro and anti‐RdRp antibodies. As control of the fractionation procedure, antibodies against the cellular cytosolic GAPDH and membrane‐associated cadherins were also used. The positions and sizes in kilodalton of the molecular weight markers are shown at the left side of each picture.