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. 2020 Apr 16;15(4):e0231223. doi: 10.1371/journal.pone.0231223

Fig 5. Effect of Dox and PYR-41 on the levels of H2B histones detected in the cytoplasm of DCs.

Fig 5

(A) Cells treated with the drug(s) indicated in the Figure for 2 hrs were fixed and labeled with a monoclonal anti-H2B antibody (green) and the DNA was stained by PI (red). Representative confocal microscopic images are shown. Instrument settings and settings of image analyses performed by ImageJ were identical for each compared sample of a particular experiment. More images are shown in S9 Fig. (B) Quantification of fluorescence microscopic images of panel A. The total nuclear H2B immunofluorescence was determined as described in Materials and Methods and normalized to the total cellular immunofluorescence. Error bars represent SEM.* indicates significant difference based on the two-tailed Student’s t-test (p<0.001).