Skip to main content
. Author manuscript; available in PMC: 2020 Apr 16.
Published in final edited form as: Thorax. 2019 Apr 29;74(7):675–683. doi: 10.1136/thoraxjnl-2018-212529

Figure 2.

Figure 2

Assessment of PM burden in AM. Wright-stained cytospin preparations of bronchoalveolar cells (BAC) (120 000 BACs per cytospin) from 30 participants (CC #) were examined to assess the proportions of AM containing PM (%AM with PM) (A) and the mean and minimum and maximum areas of the total AM area occupied by PM (PM load per AM) (B) in each study participant. Digital colour images of 50 alveolar macrophages per study participant were obtained using 1000× magnified digital bright field microscopy (Olympus BX51 microscope, Olympus, Waltham, MA). PM load per AM (areas of AM occupied by PM) was determined using ImageJ V.1.49. The areas occupied by PM within each of the AMs are expressed as per cent of the total (100%) AM area. The grey bars represent the study participants whose BACs were assessed by enzyme-linked immune absorbent spot (ELISPOT) (see figures 3–5). AM, alveolar macrophage; PM, particulate matter.