Figure 4. Effect of puerarin on epithelial-mesenchymal transition (EMT) regulators. A, Immunofluorescent staining was used to detect EMT regulator (Snail and Slug) expression in hepatocellular carcinoma (HCC) cells (magnification bars: 20 μm). B and C, Immunohistochemistry analysis (IHC) of Snail and Slug expression was performed on the same hepatocellular subcutaneous xenograft (magnification bars: 100 μm). D, Western blotting confirmed the expression change of EMT regulators in HCC cells. Data are reported as means±SD.*P<0.05, **P<0.01, ***P<0.001 compared to control (t-test). Ctr: control.
