Figure 6. miR-21 mimic and PTEN inhibitor reversed the puerarin-inhibited invasion and metastasis. A and B, Representative images of wound-healing assay and corresponding statistical analysis (magnification bars: 100 μm). Overexpression of miR-21 and PTEN inhibitor markedly reversed cell motility, which was inhibited by puerarin. C and D, Expressions of E-cadherin, N-cadherin, vimentin, snail, and slug were assessed by western blotting and qRT-PCR after overexpressing of miR-21 and inhibition of PTEN. E, Immunofluorescence analysis of vimentin expression (magnification bars: 100 μm). Data are reported as means±SD from three independent experiments. *P<0.05; **P<0.01, ***P<0.001 (t-test). Ctr: control.