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| Score | Staining intensity | Proportion of positive cells |
|---|---|---|
| 0 | Negative | <5% |
| 1 | Weak | 5−25% |
| 2 | Moderate | 26−50% |
| 3 | Strong | 51−75% |
| 4 | NA | >75% |
Staining score = staining intensity × proportion of positive cells (0−12). IHC results of miRNA-21 were scored as follows: 0, staining value 0−3; 1, staining value 4−6; 2, staining value 7−9; 3, staining value 10−12. NA: not applied. IHC results were scored by two experienced pathologists.
