| Antibodies |
| Alexa Fluor 647 Anti-mouse IgG |
Thermo Fisher Scientific |
Cat# A-31571; RRID:AB_162542 |
| Alexa Fluor 647 Anti-Rabbit IgG |
Abcam |
Cat# ab150075; RRID:AB_2752244 |
| Anti-A1arp−/vlsE−
|
This paper |
N/A |
| Anti-A1arp+/vlsE−
|
This paper |
N/A |
| Anti-A1arp+/vlsE+
|
This paper |
N/A |
| Anti-Arp antibody |
This paper |
N/A |
| Anti-BosR IgG |
General Biosciences |
Cat# AB76001-200; Clone 2F9-D2. |
| Anti-FlaB antibody |
Rockland |
Cat# 200-401-C14; RRID:AB_10703395 |
| Anti-mouse IgG |
Abcam |
Cat# ab6789; RRID:AB_955439) |
| Anti-rabbit IgG |
Abcam |
Cat# ab6802; RRID:AB_955445 |
| Anti-VlsE IgG |
Rockland |
Cat# 200-401-C33; RRID:AB_10924424 |
| Bacterial and Virus Strains |
|
B. burgdorferi A1arp−/vlsE+
|
This paper |
N/A |
|
B. burgdorferi A1arp+/vlsE−
|
This paper |
N/A |
|
B. burgdorferi A1arp+/vlsE+
|
This paper |
N/A |
|
B. burgdorferi A1arp+/vlsE− pBSV2-pncA
|
This paper |
N/A |
|
B. burgdorferi B31-A1 (A1arp−/vlsE−) |
Elias et al., 2002 |
N/A |
|
B. burgdorferi B31-A3 |
Elias et al., 2002 |
N/A |
|
E. coli Rosetta (DE3) pLysS |
Novagen |
Cat# 70956 |
| Chemicals, Peptides, and Recombinant Proteins |
| Amphotericin B |
Sigma |
Cat# A9528: CAS: 1397-89-3 |
| Betadine Surgical Scrub |
Purdue Products |
Cat# 516163 |
| Blocking buffer |
Abcam |
Cat# ab126587 |
| Clarity Western ECL |
Bio-Rad Laboratories |
Cat# 170-5060 |
| Gentamycin |
Sigma |
Cat# G1264; CAS: 1405-41-0 |
| Isopropyl β-D-1-thiogalactopyranoside |
Novagen |
Cat# 70527-3; CAS: 367-93-1 |
| Kanamycin |
Sigma |
Cat# K1377; CAS: 25389-94-0 |
| Methanol |
Sigma |
Cat# 34860 |
| Oriole Fluorescent Gel Stain |
Bio-Rad Laboratories |
Cat# 1610496; |
| Phosphomycin |
Sigma |
Cat# P5369; |
| Prolong Gold Antefade |
Thermofisher Scientific |
Cat# P36941
|
| Rifampicin |
Sigma |
Cat# R3501; CAS: 13292-46-1 |
| Syto 9 |
Thermofisher Scientific |
Cat# S34854
|
| Titermax Gold |
Sigma |
Cat# T2684 |
| Triton X-114 |
Sigma |
Cat# X-114; CAS: 9036-19-5 |
| Tyrode’s Solution |
Boston Bioproducts |
Cat# PY-921 |
| VlsE Control Protein |
Rockland |
Cat# 000-001-C33 |
| Critical Commercial Assays |
| Coomasie Plus Assay Kit |
Thermofisher Scientific |
Cat# 23236 |
| Melon Gel IgG Purification Kit |
ThermoScientific |
Cat# P145206 |
| Ni-NTA Purification System |
Thermofisher Scientific |
Cat# K95001 |
| Wizard Genomic DNA Purification Kit |
Promega |
Cat# A1120, |
| Experimental Models: Organisms/Strains |
| Mouse: C3H/HeJ |
The Jackson Laboratory |
Stock# 000659; RRID:IMSR_JAX:000659 |
| Mouse: C3SnSmn.CB17-Prkdcscid/J |
The Jackson Laboratory |
Stock# 001131; RRID:IMSR_JAX:001131 |
| Oligonucleotides |
| Table S3 |
|
|
| Recombinant DNA |
| pBSV2 |
Stewart et al., 2001 |
N/A |
| pBSV2g |
Elias et al., 2003 |
N/A |
| pBSV2g-arp
|
This paper |
N/A |
| pBSV2-pncA
|
This paper |
N/A |
| pBSV2-vlsE
|
This paper |
N/A |
| Software and Algorithms |
| FCS Express version 6 |
DeNovo Software |
https://denovosoftware.com/installation-instructions/installing-previous-versions/ |
| InCyte (GuavaSoft 3.1.1). |
Luminex Corporation |
https://www.luminexcorp.com/guava-easycyte-software/ |
| Other |
| ChemiDoc Touch Imaging System |
Bio-Rad Laboratories |
https://www.bio-rad.com/en-us/category/chemidoc-imaging-systems?ID=NINJ0Z15 |
| Guava EasyCyte 8HT Flow Cytometry |
Millipore |
https://expert.cheekyscientist.com/reviews-section/milliporeguava-easycyte-8-10ht/ |
| Leica TCS SP8 Confocal system |
Leica |
https://www.leica-microsystems.com/ |
| Nitrocellulose Membrane |
Bio-Rad Laboratories |
Cat# 1620215 |
| Probe On Plus Slides |
Fisher Scientific |
Cat# 22-230-900 |
