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. Author manuscript; available in PMC: 2020 Apr 16.
Published in final edited form as: J Am Coll Cardiol. 2017 Jul 4;70(1):115–117. doi: 10.1016/j.jacc.2017.04.055

FIGURE 1. The Effect of wt-CaM and CPVT CaM Mutants on Activity of Human RyR2.

FIGURE 1

RyR2 is in the presence of 100 nmol/l cytoplasmic Ca2+ and 2 mmol/l ATP and 0.1 mmoL/l Luminal [Ca2+] with membrane potential −4 0 mV. (A) Relative effect of wt-CaM on RyR2 open probability (P0) compared with that of N54I and N9BS (mean ± SEM with replicate numbers in each bar). P0, (−CaM) denotes mean absolute P0 before wt or mutant CaM application. **Significant difference to 1 (p < 0.01). (B) Western blots of RyR2 subjected to incubations in PP1 (15 min) or PKA (5 min) at 30°C probed with antibodies for PS2808 (left), stripped and reprobed with antibodies for RyR2 (right). Ca2+ = calcium ion; CaM = calmodulin; CPVT = catecholaminergic polymorphic ventricular tachycardia; PKA = protein kinase A; PP1 = protein phosphatase 1; RyR2 = ryanodine receptor 2; wt = wild-type.