Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Arabidopsis thaliana) | SBT3.8 | The ArabidopsisInformation Resource (TAIR) | At4g10540 | amplified from genomic DNA |
| Strain, strainbackground (Escherichia coli) | BL21-RIL | Agilent | ||
| Strain, strainbackground (Escherichia coli) | BL21 pEVOL SYRS | Liu et al., 2009 | ||
| Genetic reagent(Arabidopsis thaliana) | sbt3.8 | Nottingham Arabidopsis Stock Center | SALK_052039 | |
| Genetic reagent(Arabidopsis thaliana) | tpst-1 | Nottingham Arabidopsis Stock Center | SALK_009847 | |
| Genetic reagent(Arabidopsis thaliana) | PCLEL6:EPI1a; PCLEL9:EPI1a | this paper | Transgenic lines expressingEPI1a under control of theCLEL6or CLEL9 promoter | |
| Genetic reagent (Arabidopsis thaliana) |
PCLEL6:EPI10; PCLEL9:EPI10 | this paper | Transgenic lines expressingEPI10 under control of theCLEL6 or CLEL9 promoter | |
| Genetic reagent(Agrobacterium tumefaciens) | C58C1; GV3101 | Community resource | NCBI:txid176299 | GV3101 with and withoutpSOUP helper plasmid |
| Antibody | anti-GFP, polyclonal, rabbit | Thermo Fisher Scientific | Cat# A-11122 | 1:10000 |
| Antibody | anti-His, monoclonal, mouse | Dianova | Cat# DIA-900–200 | 1:10000 |
| Antibody | anti-FLAG-HRP, monoclonal, mouse | Sigma-Aldrich | Cat# A8592 | 1:5000 |
| Antibody | Goat-anti-rabbit IgG, HRP conjugate | Calbiochem | Cat# 401315 | 1:10000 |
| Antibody | Goat-anti-mouse IgG, HRP conjugate | Calbiochem | Cat# 402335 | 1:10000 |
| Recombinant DNA reagent | pCR2.1-Topo | Life Technologies | Cat# K4510-20 | PCR cloning vector |
| Recombinant DNA reagent | pART7, pART27 | Gleave, 1992 | plant transformationvectors | |
| Recombinant DNA reagent | sfGFP | Pédelacq et al., 2006 | used as template for cloning | |
| Recombinant DNA reagent | pETDuet1 | Novagen/Merck | Cat# 71146 | for expression of recombinant proteins |
| Recombinant DNA reagent | pMS119EH-sfGFP | Pross et al., 2016 | ||
| Recombinant DNA reagent | EPI1a, EPI10 | Schardon et al., 2016 | codon-optimized, with plant signal peptide | |
| Recombinant DNA reagent | pGreen0229 | Hellens et al., 2000 | plant transformation vector | |
| Recombinant DNA reagent | VMA12-mRFP | Viotti et al., 2013 | ER marker | |
| Recombinant DNA reagent | VHP1Pro:ManI-mCherry | this paper | Golgi marker; see Materialsand methods section andSupplementary file 2 | |
| Recombinant DNA reagent | VHP1Pro:ST-mCherry | this paper | Golgi marker; see Materialsand methods section andSupplementary file 2 | |
| Recombinant DNA reagent | Sec | this paper | sfGFP-tagged CLEL6/9expression constructs; Figure 2 | |
| Recombinant DNA reagent | KDEL | this paper | sfGFP-tagged CLEL6/9expression constructs withER retention signal; Figure 2 | |
| Recombinant DNA reagent | XylT | this paper | sfGFP-tagged CLEL6/9expression constructs withXylT membrane anchor; Figure 2 | |
| Recombinant DNA reagent | Secm | this paper | sfGFP-tagged CLEL6/9 expression constructs, SBT6.1 cleavage sitesmutated; Figure 2 |
|
| Recombinant DNA reagent | Δ-Sec | this paper | sfGFP-tagged CLEL6/9 pro-domain deletion constructs; Figure 3 | |
| Recombinant DNA reagent | Δ-KDEL | this paper | sfGFP-tagged CLEL6/9prodomain deletionconstructs with ER retention signal; Figure 3 | |
| Recombinant DNA reagent | Δ-XylT | this paper | sfGFP-tagged CLEL6/9 prodomain deletionconstructs with XylTmembrane anchor; Figure 3 | |
| Recombinant DNA reagent | SBT3.8-sfGFP | this paper | Expression construct forSBT3.8 C-terminally fusedwith sfGFP; Figure 5 | |
| Peptide, recombinant protein | SBT3.8 | this paper | Recombinant, His-taggedSBT3.8, purified from N. benthamiana cell wall extracts | |
| Peptide, recombinant protein | CLEL6 | PepMic | DsYPQPHRKPPIHNE | |
| Peptide, recombinant protein | CLEL9 | PepMic | DMDsYNSANKKRPIHNR | |
| Peptide, recombinant protein | eCLEL6 | PepMic | GEEVVVMDYPQPHRKPPIHNE | |
| Peptide, recombinant protein | proCLEL6; CLEL6 precursor | this paper | purified from E. coli BL21-RIL cells | |
| Peptide, recombinant protein | CLEL6-D71A | this paper | Site-directed mutant of the CLEL6precursor purified from E. coli BL21-RILcells | |
| Peptide, recombinant protein | sulfoCLEL6 | this paper | purified from E. coliBL21-pEVOL SYRScells | |
| Commercial assay or kit | Ni-NTA agarose | Qiagen | Cat# 30210 | |
| Software, algorithm | GraphPad Prism | Graphpad | preparation of figures andstatistical analyses |
|
| Software, algorithm |
ImageJ | ImageJ | analysis ofgravitropic response |