Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene Mus musculus | nestin | MGI:101784 NCBI Gene: 18008 |
||
| Strain, strain background Mus musculus | C57Bl/6J | The Jackson Laboratory | JAX Stock no. 000664 RRID:IMSR_JAX:000664 |
Females |
| Strain, strain background Mus musculus | CD1 | Obtained from the Baylor College of Medicine Center for Comparative Medicine | ||
| Strain, strain background Mus musculus | Nestin-CreER | Obtained from Dr. Mirjana Maletic-Savatic Lab at Baylor College of Medicine | Cryopreserved by Andreas Tolias Lab at Baylor College of Medicine | |
| Strain, strain background Mus musculus | Ai9 | The Jackson Laboratory | JAX Stock no. 007909 RRID:IMSR_JAX:007909 |
|
| Antibody | Rabbit anti-Tbr2 | Abcam | cat. no. AB23345 RRID:AB_778267 |
|
| Antibody | Mouse anti-Pax6 | Developmental Studies Hybridoma Bank (DSHB) at the University of Iowa. PAX6 was deposited to the DSHB by Kawakami, A. | DSHB Hybridoma Product PAX6 RRID:AB_528427 |
|
| Antibody | Goat anti-tdTomato | Sicgen | cat. no. AB8181-200 RRID:AB_2722750 |
|
| Chemical compound, drug | Tamoxifen (≥99%) | Sigma-Aldrich | cat. no. T5648 | |
| Chemical compound, drug | Progesterone (≥99%) | Sigma-Aldrich | cat. no. P0130 | |
| Other | Corn Oil | Sigma-Aldrich | cat. no. C8267 | |
| Other | DNA-OFF | Clontech | Cat. no 9036 | |
| Other | RNase Zap | Thermo Fisher Scientific | Cat. no. AM9780 | |
| Peptide, recombinant protein | Recombinant RNase inhibitor | Clontech | 2313A | |
| Other | Potassium D-gluconate (K-gluconate,≥99%) | Sigma-Aldrich | cat. no 64500 | |
| Other | Potassium chloride (KCl, for molecular biology,≥99.0%) | Sigma-Aldrich | cat. no. P9541 | |
| Other | HEPES solution (1 M, BioReagent) | Sigma-Aldrich | cat. no. H3537 | |
| Other | Ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, for molecular biology,≥97.0%) | Sigma-Aldrich | cat. no. E3889 | |
| Other | Adenosine 5’-triphosphate magnesium salt (Mg-ATP,≥95%) | Sigma-Aldrich | cat. no. A9187 | |
| Other | Guanosine 5’-triphosphate sodium salt hydrate (Na-GTP,≥90%) | Sigma-Aldrich | cat. no. 51120 | |
| Other | Phosphocreatine disodium salt hydrate (Na2-phosphocreatine,≥97%) | Sigma-Aldrich | cat. no. P7936 | |
| Other | Glycogen (RNA grade) | Thermo Fisher Scientific | cat. no. R0551 | |
| Other | Biocytin (≥98%) | Sigma-Aldrich | cat. no. B4261 | |
| Sequence-based reagent | ERCC RNA spike-in mix | Thermo Fisher Scientific | cat. no. 4456740 | sequences available at website |
| Other | Tris-EDTA buffer solution (TE buffer, BioUltra, for molecular biology) | Sigma-Aldrich | cat. no. 93283 | |
| Other | Triton X-100 | Sigma-Aldrich | cat. no. T8787 | |
| Other | Betaine (BioUltra,≥99.0%) | Sigma-Aldrich | cat. no. 61962 | |
| Other | dNTPs (25 mM each) | Thermo Fisher Scientific | cat. no. R1121 | |
| Peptide, recombinant protein | Superscript II reverse transcriptase (SSIIRT) | Thermo Fisher Scientific | cat. no. 18064014 | |
| Other | MgCl2 (1M, molecular biology grade) | Thermo Fisher Scientific | cat. no. AM9530G | |
| Commercial assay, kit | KAPA Biosystems HiFi HotStart Ready Mix | Thermo Fisher Scientific | cat. no. NO0295239 | |
| Other | TAPS (≥99.5%) | Sigma-Aldrich | cat. no. T5130 | |
| Other | Polyethylene glycol solution (PEG-8000, 40% wt/vol) | Sigma-Aldrich | cat. no. P1458 | |
| Commercial assay, kit | Nextera XT index kit v2 set A for 96 indices, 384 samples | Illumina | cat. no. FC-131–2001 | |
| Commercial assay, kit | Axygen AxyPrep mag PCR clean-up kit | Thermo Fisher Scientific | cat. no. 14223151 | |
| Commercial assay, kit | KAPA HiFi PCR Kit | KAPA Biosystems | cat. no. KK2103 | |
| Other | Hoechst 33342 | Invitrogen | cat. no. H3570 | |
| Other | Antigen unmasking solution | Vector Laboratories | cat. no. H-3300 | |
| Other | Vectashield antifade mounting medium | Vector Laboratories | cat. no. H-1000 | |
| Sequence-based reagent | Oligo-dT30VN (HPLC purified) | Biomers.net | oligonucleotide | 5’- AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’, where V represents A, C or G and N represents any nucleotide |
| Sequence-based reagent | IS PCR Oligo (HPLC purified) | Biomers.net | oligonucleotide | 5’-AAGCAGTGGTATCAACGCAGAGT-3’ |
| Sequence-based reagent |
Template switching oligonucleotide (LNA-TSO; RNase-free; HPLC purified | Exiqon | oligonucleotide | 5’- AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3’, where rG indicates riboguanosines and +G indicates a locked nucleic acid (LNA)-modified guanosine |
| Software, algorithm | Patchmaster software | HEKA | RRID:SCR_000034 | |
| Software, algorithm | STAR v2.4.2a | https://github.com/alexdobin/STAR/releases/tag/STAR_2.4.2a | RRID:SCR_015899 | |
| Software, algorithm | Neurolucida | MBF Bioscience | RRID:SCR_001775 | |
| Other | Glass capillaries (2.0 mm OD, 1.16 mm ID) | Sutter Instruments |