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. Author manuscript; available in PMC: 2021 Apr 3.
Published in final edited form as: J Proteome Res. 2020 Feb 27;19(4):1635–1646. doi: 10.1021/acs.jproteome.9b00840

Figure 4.

Figure 4.

Validation of SAAV sites for variant peptides derived from KRAS, SLC37A4, and TP53 using SRM. (A) Variant peptide LVVVGADGVGK (variant peptide G12D) from KRAS. (B) Canonical peptide LVVVGAGGVGK from KRAS. (C) Variant peptide FVSGVLSDQMSAR from SLC37A4. (D) Variant peptide NSFEVHVCACPGR (variant peptide R273H) from TP53. The variant peptide from SLC37A4 was detected by PRISM-SRM. The other three peptides were detected by regular LC-SRM. The endogenous peptides were confirmed by matching their corresponding heavy internal standards in the retention time and the SRM peak patterns. The top panel shows the SRM signal for endogenous peptides; the bottom panel shows the SRM signal for heavy internal standards (13C6, 15N2 on the C-terminal K or R). IS, internal standard.