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Sample Fractionation and Mass Spectrometry Analysis
| sample set | fractionation method | number of fractions | MS/MS analysis | number of runs per set |
|---|---|---|---|---|
| sets 1–5 | Pierce high-pH reversed-phase peptide fractionation kit | 9 | LC-MS/MS | 9 |
| sets 6–7 | low-pH nano-RPLC | 11 | CZE-MS/MS | 11 |
| 17 | LC-MS/MS | 17 | ||
| sets 8–13 | Pierce high-pH reversed-phase peptide fractionation kit | 10 | LC-MS/MS | 10 |
