Table 2.
Number of SAAVs Identified Using Three Different Searching Algorithmsa
| SEQUEST algorithm against Swiss-CanSAAVs database | MetaMorpheus against RNA-seq database | Mascot algorithm and SAVControl | ||||
|---|---|---|---|---|---|---|
| samples | proteins with SAAVs | peptides with SAAVs | proteins with SAAVs | peptides with SAAVs | proteins with SAAVs | peptides with SAAVs |
| sets 1–5 | 293 | 326 | 171 | 190 | 158 | 172 |
| sets 6–7 | 118 | 123 | 58 | 64 | 14 | 15 |
| sets 8–13 | 113 | 123 | 71 | 77 | 50 | 53 |
| total | 382 | 197 | 165 | |||
| percentage of all SAAVs (%) | 78.1 | 40.9 | 34.2 | |||
SEQUEST algorithm in software Proteome Discoverer (PD) 1.4 against Swiss-CanSAAVs database, MetaMorpheus against the customized RNA-seq database, and Mascot algorithm coupled with SAVControl quality control.