| Construction of deficient mutants |
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nox |
nox_A-forward |
CAACCTGCAGTTTTTGCTGTTGATTAATATGTTTGAAAAT |
Pst I |
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nox_A-reverse |
TGTGAAGGAGTGTTTAACTATCCATTCGAATTGCAAACAA |
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nox_B-forward |
TTGTTTGCAATTCGAATGGATAGTTAAACACTCCTTCACA |
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nox_B-reverse |
TTC GAAGCTTGTTATCCGCAACGTGCCGT |
Hind III |
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nox5 |
nox5_A-forward |
CCATGGATCCCCCGTGAAGCGTAGTTGTTG |
BamH I |
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nox5_A-reverse |
ACGAATTCATAATTTTCCCCCAGCATCTGCCTTCCTTTCA |
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nox5_B-forward |
TGAAAGGAAGGCAGATGCTGGGGGAAAATTATGAATTCGT |
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nox5_B-reverse |
GCC AAAGCTTCTTGATCGGCTCGTCTGATC |
Hind III |
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poxF |
poxF_A-forward |
GTTGGGATCCAGCCAATGGCGACTTCTGGA |
BamH I |
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poxF_A-reverse |
ACTTTTTGGGAGGGATTCTTCTAGTGATTAAAAAAGAGAT |
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poxF_B-forward |
ATCTCTTTTTTAATCACTAGAAGAATCCCTCCCAAAAAGT |
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poxF_B-reverse |
TGCACTGCAGGGCTTGGCAGTGCCGAA |
Pst I |
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cidC |
cidC_A-forward |
GCTAGGATCCCAGCGTGACGGCTTTTTATA |
BamH I |
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cidC_A-reverse |
TGATACAAGCTAATCGAAAAATCAAAATCTCCTTTATCGC |
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cidC_B-forward |
GCGATAAAGGAGATTTTGATTTTTCGATTAGCTTGTATCA |
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cidC_B-reverse |
GACAAAGCTTGGGACACAATATGCTGAGGC |
Hind III |
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ahpC |
ahpC_A-forward |
GCTAGGATCCGCTACATTCTCGATATCGGT |
BamH I |
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ahpC_A-reverse |
CTAAAAATGGAGGTAATATCAACGGTGCTTTGATCAGCTA |
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ahpC_B-forward |
TAGCTGATCAAAGCACCGTTGATATTACCTCCATTTTTAG |
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ahpC_B-reverse |
GATTAAGCTTATGTCCGTGATCGTCCGTTTATTAAGAATC |
Hind III |
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ohrR |
ohrR_A-forward |
CAAGCTGCAGGAGCGATGATGCCTAG |
Pst I |
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ohrR_A-Reverse |
CCTTATTTTTGGGCGGCTGCTTCCTCCTAA |
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ohrR_B-forward |
TTAGGAGGAAGCAGCCGCCCAAAAATAAGG |
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ohrR_B-Reverse |
GCACAAGCTTAAGGTTGATGGATCAGGATG |
Hind III |
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sod |
sod_A-forward |
CTGACTGCAGCGCTGAAATTGCGCAAAATC |
Pst I |
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sod_A-reverse |
AGTGACCAAACATCGAGGAATCAACCTTTC |
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sod_B-forward |
GAAAGGTTGATTCCTCGATGTTTGGTCACT |
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sod_B-reverse |
GGTTAAGCTTGGTAAATTACCATCCCCAAAAGGTG |
Hind III |
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suf |
suf_A-forward |
AATAGGATCCTTGTGGTCCAAGCATCAGGAC |
BamH I |
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suf_A-reverse |
TTGGAGGCAAGTGTCCTGGTTGACTTAAAA |
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suf_B-forward |
TTTTAAGTCAACCAGGACACTTGCCTCCAA |
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suf_B-reverse |
GCATCTGCAGAATCATGCTAAAATGTTGGC |
Pst I |
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flp |
flp_A-forward |
GCTAGGATCCCAAGCACAGACCCATTTTG |
BamH I |
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flp_A-reverse |
CATCACTCGGCAACCGTTGCCACCTCCTAA |
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flp_B-forward |
TTAGGAGGTGGCAACGGTTGCCGAGTGATG |
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flp_B-reverse |
TCGGCTGCAGGCCCGATGACCTCAAC |
Pst I |
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dpsB |
dpsB_A-forward |
GGTCGGATCCAATGCCTTACGGTTACGGCA |
BamH I |
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dpsB_A-reverse |
CGCTCTGTCAACAAGCTTGAGTTCTTCTCCTCTTAACCGC |
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dpsB_B-forward |
GCGGTTAAGAGGAGAAGAACTCAAGCTTGTTGACAGAGCG |
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dpsB_B-reverse |
GCGGCTGCAGGAGAAGCAATTGTTGAAAGTCAGT |
Pst I |
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cydAB |
cydAB_A-forward |
CCTCGGATCCCCTACTCACTATCATTC |
BamH I |
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cydAB_A-reverse |
TCTTTTCGCCGCCGCTTTGATCACCTCTAT |
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cydAB_B-forward |
ATAGAGGTGATCAAAGCGGCGGCGAAAAGA |
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cydAB_B-reverse |
TAGACTGCAGCCTGAACACGTGCATACTG |
Pst I |
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gshR1 |
gshR-1_A-forward |
CAATGTCGACGCTTCGGTATCCCGG |
Sal I |
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gshR-1_A-reverse |
ACAAGGAGGATCAATGGTTGTAGCGGCAAT |
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gshR-1_B-forward |
ATTGCCGCTACAACCATTGATCCTCCTTGT |
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gshR-1_B-reverse |
GTTGAAGCTTTGATCGGCGCGCCG |
Hind III |
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gshR2 |
gshR-2_A-forward |
AACAGGATCCCAATTAAGGTCATATCATCCC |
BamH I |
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gshR-2_A-reverse |
CACAGAGACGAAGGAAGGCGTAATTGATAC |
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gshR-2_B-forward |
GTATCAATTACGCCTTCCTTCGTCTCTGTG |
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gshR-2_B-reverse |
GCGGGTCGACGCATCACGGCCTAAG |
Sal I |
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ipr |
ipr_A-forward |
CCGACTGCAGCGTATTAATCACGTTGC |
Pst I |
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ipr_A-reverse |
TTTGGAGGCGTAACGATAAAGTTGCTTGAT |
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ipr_B-forward |
ATCAAGCAACTTTATCGTTACGCCTCCAAA |
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ipr_B-reverse |
GGCCAAGCTTATGAGGTACAGCTTCGC |
Hind III |
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npr |
npr_A-forward |
CAATGGATCCATCGGAGCATATCCCTTCAG |
BamH I |
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npr_A-reverse |
AATTAGGAGGAATTTACTTTTAAAAAGACA |
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npr_B-forward |
TGTCTTTTTAAAAGTAAATTCCTCCTAATT |
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npr_B-reverse |
GATACTGCAGATTTGGCCGGGACAAGTG |
Pst I |
| Confirmation of deficient mutants |
|
nox |
279962_F |
GTAGCATCGGCAATTGTCATGTAGTGTCAC |
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282934_R |
CTGTTTTGAGTCATACCGTGCAACCCG |
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nox5 |
177892_F |
CTGCGGTTCGATGGTGCTAAGGTCACCTTC |
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180877_R |
GTTTTGACGCATTCATCGAATCGAGTCGCG |
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poxF |
2268013_F |
GTCTGACTAATATGCAGTGGCGCAAAGTGAG |
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2270930_R |
CGAGGCAGCCAAAGCTTTCGTTAAGAAGCAC |
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cidC |
498129_F |
CGTTGCTTCGATCATGGTCTGGCAGAATTC |
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501457_R |
GGCCAGTGGCATTCCTGATTACACCGAG |
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ahpC |
2611951_F |
GAATAACCATAGAAAGAAGGGAGGCAGTTG |
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2614120_R |
AATTATTACCAGCCGGACCCGAGCACAAAG |
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ohrR |
1040892_F |
CAATTTTAGATCCGGATACCATGGCGATTTC |
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1043371_R |
CTCCATTGCACACAAATTGCACACAAATTC |
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sod |
2004800_F |
CAATCGCATGCTCGGAAATGAGTTTCAAAC |
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2007409_R |
GGAAATAGGTATGCGATATTCATTTACGAC |
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flp |
69484_F |
CTTATGGAGGAGGTTTCGATCCTATAGAAC |
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71774_R |
GCAGTATACCAACGTTCCAACCGCTATC |
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dpsB |
68755_F |
GAAAAGGTGATGTTTGTCGGTGACGGGATC |
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70973_R |
GTATTTAAAAAACATCACTCGGCAACCTCACCAAG |
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cydAB |
14573_F |
GAAGCTTAGAGTGACGGCTAATGAAC |
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18661_R |
CCGCAAAATGGACGGGTATTATCCATC |
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gshR1 |
2311599_F |
CAATGGGTTGCGGTTCGCATTCCTGAC |
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2314729_R |
CTGTCGGAACGTTACTCGTCATGCTTG |
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gshR2 |
2748254_F |
CAGTGACCAAAGATTTTGACCATCATAAAC |
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2751189_R |
GTTGATCCAACGAGCGGCGTCATC |
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ipr |
706255_F |
GGGTAATAAACCAGCAATGACCACAAGACG |
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708795_R |
CTAGAATTCAATCGAAATAATATTCGGATTGTCGG |
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npr |
464266_F |
CCAATTTTTTCTGCAAAGTCCTTTTGAGAG |
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467233_R |
CGTTTTACAAGCATGGGAAAATACGGC |
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| qRT-PCR |
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NADH peroxidase |
ACGGCAATCCACAAGTTTGC |
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TTGTTGTTGAACGGCGAGTG |
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Elongation factor Tu |
AACCGCGAACAAGTTGAACG |
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ACGGCCACCTTCTTCTTTTG |
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Glyceraldehyde 3-phosphate dehydrogenase |
AACACGATTCCTCACAGCAC |
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ACAACAGAAACACGCTGTGC |
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