Fig. 2.

Growth of viable cells in artificial intestinal juice.

Cells treated for 2 hr with acid-pepsin as previously described were added to MRS medium containing 0.3% oxgall powder. The pH was adjusted to 7.0 using 5N-NaOH. The cells were cultured at 37°C under anaerobic conditions for 18 hr. The growth rate of viable cells was calculated from the increase in the number of viable bacteria after 2 hr treatment with acid-pepsin. Straight lines above bars indicate the standard deviation (n=3). Pediococcus strain was later identified as P. pentosaceus QU 19.