KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-LAB-1 | [15] | N/A |
| guinea pig anti-HTP-3 | [51] | N/A |
| rabbit anti-phospho-Histone H3 (Ser10) | Millipore | Cat# 06-570; RRID: AB_310177 |
| rat anti-SMC-1 | [52] | N/A |
| mouse anti-GFP | Roche | Cat# 11814460001; RRID: N/A |
| donkey anti-rabbit Cy5 | Jackson Immunoresearch | Cat# 711-175-152; RRID: AB_2340607 |
| donkey anti-guinea pig Alexa488 | Jackson Immunoresearch | Cat# 706-545-148; RRID: AB_2340472 |
| donkey anti-rabbit Cy3 | Jackson Immunoresearch | Cat# 711-165-152; RRID: AB_2307443 |
| goat anti-mouse Alexa488 | Jackson Immunoresearch | Cat# 115-546-146; RRID: AB_2338868 |
| donkey-anti-guinea pig Cy3 | Jackson Immunoresearch | Cat# 706-165-148; RRID: AB_2340460 |
| mouse anti-tubulin | Sigma Aldrich | Cat# T0926; RRID: N/A |
| rabbit anti-RAD-51 | Novus Biological (SDI) | Cat# 29480002; RRID: AB_2616441 |
| Bacterial and Virus Strains | ||
| Ahringer RNAi library clone: pL4440-W06D4.6 | Source Bioscience | N/A |
| Ahringer RNAi library clone: pL4440-F54C8.3 | Source Bioscience | N/A |
| Ahringer RNAi library clone: pL4440 | Source Bioscience | N/A |
| cosmid for chromosome III FISH probe: T03F6 (III, right) | Sanger Center | WB ID: T03F6 |
| cosmid for chromosome III FISH probe: cosmid F11F1 (III, right) | Sanger Center | WB ID: F11F1 |
| cosmid for chromosome III FISH probe: cosmid T28A8 (III, right) | Sanger Center | WB ID: T28A8 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fluorescein-12-dCTP | Perkin Elmer | Cat# NEL-424 |
| Cyanine 3-CTP | Perkin Elmer | Cat# NEL580001EA |
| Poly-L-Lysine (0.01%) | Sigma | Cat# P8920 |
| CytoBond | SciGene | Cat# 2020-00-1 |
| DAPI | Thermo Fisher Scientific | Cat# D1306 |
| VectaShield | Vector Laboratories | Cat# H-1000 |
| TOPO® TA Cloning® kit | Thermo Fisher Scientific | Cat#451641 |
| Experimental Models: Organisms/Strains | ||
| N2 (wild type) | https://cgc.umn.edu/strain/N2 | WB ID: WBStrain00000001 |
| CV620: F14E5.8(ttTi5605); spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (II;IV;V) | this paper | N/A |
| AV630: meIs8[unc-119(+) Ppie-1::gfp::cosa-1] (II) | [26] | WB strain: AV630 WB ID: WBStrain00000296 |
| CV451: meIs8[unc-119(+) Ppie-1::gfp::cosa-1]; T07E3.4(ttTi23808); spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (II;III;IV;V) | this paper | N/A |
| CV613: meIs8[unc-119(+) Ppie-1::gfp::cosa-1]; ttTi13055; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (II;III;IV;V) | this paper | N/A |
| CV614: meIs8[unc-119(+) Ppie-1::gfp::cosa-1]; ttTi17604; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (II;III;IV;V | this paper | N/A |
| CV686: meIs8[unc-119(+) Ppie-1::gfp::cosa-1]; spo-11(ok79) IV/]/nT1 [unc-?(n754) let-?] (II,IV) | this paper | N/A |
| CV616: ttTi1730; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this paper | N/A |
| CV405: T07E3.7(ttTi23808); spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this paper | N/A |
| CV760: T07E3.7(ttTi23808); krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this paper | N/A |
| CV771: tTi2384; ; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this paper | N/A |
| CV561: ttTi13055; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this paper | N/A |
| CV683: ttTi17604; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;IV;V) | this study | N/A |
| CV838: T07E3.7(ttTi23808); ttTi13055; spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (III;III;IV;V) | this paper | N/A |
| AV106: spo-11(ok79) IV/nT1 [unc-?(n754) let-?] (IV) | [21] | WB strain: AV106; WB ID: WBVar00091464 |
| CV394: spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (IV;V) | this paper | N/A |
| EN909: krls14[Prom(hsp)::transposase; lin-15B; cc::GFP] | [20] | N/A |
| CV485: spo-11(ok79) IV/nT1 [unc-?(n754) let-?]; C35A5.14(ttTi35006); krls14[Prom(hsp)::transposase; lin-15B; cc::GFP]/nT1 [unc-?(n754) let-?] (IV;V;V) | this paper | N/A |
| Oligonucleotides | ||
| FISH probe for chromosome V: 5S_F: 5’-TACTTGGATCGGAGACGGCC-3’ | [21] | N/A |
| FISH probe for chromosome V: 5S_R: 5’-CTAACTGGACTCAACGTTGC-3’ | [21] | N/A |
| genotyping primer: ttTi23808_F: 5’-CTGCGCGAAGATTATCCCGACGA-3’ | this paper | N/A |
| genotyping primer: ttTi23808_R: 5’-CGCGAATCGTACTCCATCAATGC-3’ | this paper | N/A |
| genotyping primer: ttTi35006_F: 5’-CATCATGCTCCTAACACGTGTGG-3’ | this paper | N/A |
| genotyping primer: ttTi35006_R: 5’-TCTGAAAGCTCGGATATGAGCTAAC-3’ | this paper | N/A |
| genotyping primer: ttTi2384_F: 5’-GACGTAGAAATCAACTGGAC-3’ | this paper | N/A |
| genotyping primer: ttTi2384_R: 5’-AAGACTCCCATTATTTGGTC-3’ | this paper | N/A |
| genotyping primer: ttTi1730_F: 5’-TTCCCTAGTCCCATGTTTTGGTT-3’ | this paper | N/A |
| genotyping primer: ttTi1730_R: 5’-CCACTTCCCAAACCACCAAAAAT-3’ | this paper | N/A |
| genotyping primer: ttTi13055_F: 5’-CTTAAAGGCGATTTGATGCACCA-3’ | this paper | N/A |
| genotyping primer: ttTi13055_R: 5’-GGGCCTAATTATACCTAAGCCCC-3’ | this paper | N/A |
| genotyping primer: ttTi5605_F: 5’-CGGCCGAAATTTTACTTGATCGA-3’ | this paper | N/A |
| genotyping primer: ttTi5605_R: 5’-ACACCCGGGTTTGTCTAGATATG-3’ | this paper | N/A |
| genotyping primer: ttTi17604_F: 5’-CTCTCTCTGAAATGAAAACAAG-3’ | this paper | N/A |
| genotyping primer: ttTi17604_R: 5’-CTTGGACCAGTTACAACATC-3’ | this paper | N/A |
| Software and Algorithms | ||
| SoftWoRx | GE Healthcare Life Sciences | N/A |
| Priism | [4, 25, 64] | https://msg.ucsf.edu |
| XQuartz | [4, 25, 64] | https://www.xquartz.org |
| ImageJ Fiji | [53, 54, 65] | https://imagej.net/Fiji |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| R Studio | [66] |
https://www.r-project.org https://rstudio.com |