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. 2020 Mar 4;53(4):e12750. doi: 10.1111/cpr.12750

Figure 2.

Figure 2

Identification and characterization of LOC100133669. A, Schematic diagram of LOC100133669 from NCBI RefSeq and RACE. Blue boxes, exons annotated by NCBI RefSeq. Orange boxes, additional sequence and exon determined by RACE. Blue lines, introns. Arrows on blue lines indicate transcriptional directions. B, Gel electrophoresis of nested PCR products from 5′‐RACE and 3′‐RACE. Arrows indicate purpose strips. C, The full‐length sequence of LOC100133669 transcript. Blue, reference sequence from NCBI RefSeq. Orange, added sequence from RACE. D, E, ORF finder software (D) and CPAT software (E) prediction for protein‐coding potential of LOC100133669. F, Gel electrophoresis of PCR products reversed with oligo dT primer or random 6 mers, respectively, in KYSE150, KYSE410, KYSE450 and KYSE510 cells. G, Subcellular distribution of LOC100133669 in KYSE450 and KYSE510 cells. GAPDH and myc precursor RNA (pre‐myc) were served as cytoplasmic and nuclear references, respectively. Data are presented as mean ± SD, n = 3