SKA1 promotes PDAC proliferation in vitro and in vivo. A, Immunoblotting was performed in PANC‐1 and BxPC‐3 cells transfected with control shRNA (sh‐ctr) and SKA1 knock‐down shRNAs (sh‐SKA1), in Capan‐1 and SW1990 cells transfected with empty vector (vector) and lentivirus‐mediated flag‐tagged overexpression SKA1(SKA1). B, MTT assay showed the SKA1 facilitates PDAC cell growth ability, the significances were identified based on the comparison of counterpart. *P < .05. C and D, Cell cycle analysis by flow cytometry presented a significantly increased percentage of sh‐SKA1 cells in the G2/M phase, and related proteins were detected by Immunoblotting. E, The subcutaneous tumorigenic ability of tumour cells was measured (n = 5 per group). Expression of SKA1 promoted tumour growth and increased tumour weight in nude mice (P = .03). F, Percentage of positive Ki67 staining cells in tumour tissues was counted by immunohistochemical analysis. Data are presented as the mean ± SEM from three independent cell function experiments