Figure 4.

SKA1 facilitates EMT in PDAC cells. A, A significant reduction of vimentin and N‐cadherin but increase E‐cadherin was detected in PANC‐1‐shSKA1 cells compared to PANC‐1‐sh‐ctr cells by immunofluorescence staining, and vice versa in Capan‐1 cells. B, The protein expression levels of EMT markers and transcription factors (E‐cadherin, N‐cadherin, ZO‐1, Vimentin, Snail, Slug, Twist, TCF8/ZEB1) in modified PANC‐1 and Capan‐1 cells were assayed by Western blotting. GAPDH was used as an internal control