. 2020 Mar 13;104(9):3869–3884. doi: 10.1007/s00253-020-10493-3

Table 1.

Primers for qPCR and RT-qPCR studies in L. gasseri LM19 and detection of C. perfringens

Gene	Accession number	Primer	Sequence (5′–3′)	Product size	Reference/accession no.
gyrase A L. gasseri LM19	7791_LM19_00854	LGgyrAF	TTGATTGCCTTAACCCTTCG	136	This work
gyrase A L. gasseri LM19	7791_LM19_00854	LGgyrAR	TTCCCATTGAACGAACATCA	136	This work
bact_1	7791_LM19_00792	Cluster 1.1F	TATTGGTGCATGGAGAGGTG	124	This work
bact_1	7791_LM19_00792	Cluster 1.1R	CCAGCCCACACATTGTACTG	124	This work
bact_2	7791_LM19_00793	Cluster1.2F	TTGGGGTAGTGTTGCAGGAT	97	This work
bact_2	7791_LM19_00793	Cluster1.2R	TGATGTTGCAGCTCCGTTAG	97	This work
helveticin J-like	7791_LM19_00025	Cluster2F	CTTGGGTACAAAGCGGAGAA	176	This work
helveticin J-like	7791_LM19_00025	Cluster2R	GCCTGCTCGGTTAAGATAAG	176	This work
gamA (=gasT)	7791_LM19_00116	Cluster3.1F	CTGGATGGGCTCTTGGAAAT	112	This work
gamA (=gasT)	7791_LM19_00116	Cluster3.1R	TTTCCGAATCCACCAGTAGC	112	This work
gamX (=gatX)	7791_LM19_00117	Cluster3.2F	TGGGGGAATGCTGTAATAGG	100	This work
gamX (=gatX)	7791_LM19_00117	Cluster3.2R	CTCCTAAGCCACAGGCAGTC	100	This work
gamY	7791_LM19_00122	GamYF	ACTCAAATCGTAGGAGGAAAAGG	150	This work
gamY	7791_LM19_00122	GamYR	AAAGCATGCACCTGAACCA	150	This work
gamM	7791_LM19_00123	GasMF	AGCAGGAGGAGCATTTTCAA	90	This work
gamM	7791_LM19_00123	GasMR	CCTGCTGCACCACCTAAAAT	90	This work
Immunity gene gamI3	7791_LM19_00118	Cluster 3.3F	CAGATGAAGAAGCATTACTTGAAAA	102	This work
Immunity gene gamI3	7791_LM19_00118	Cluster 3.3R	TTCCAGGCCAAGTATTAGTTGTA	102	This work
C. perfringens 16S	MN960263	s-Clper-F	GGGGGTTTCAACACCTCC	170	(Nagpal et al. 2015)
C. perfringens 16S	MN960263	ClPER-R	GCAAGGGATGTCAAGTGT	170	(Nagpal et al. 2015)