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. 2020 Apr 16;10:6560. doi: 10.1038/s41598-020-63482-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CDDO-Me redirects cytokine production of F4/80⁺ TAMs in vivo. (a) Whole mammary/tumor tissue of PyMT mice fed diet with or without 50 mg CDDO-Me/kg diet for 8 weeks was digested to a single cell suspension and analyzed by multicolor flow cytometry for analysis of macrophages. Expression of surface markers is represented as percentage of cells positive for the respective marker vs. vehicle (n = 6–10 per group). (b) Mouse weights were recorded immediately prior to tissue resection and mammary/tumor tissue weights were recorded immediately after tissue resection (n = 12–14 per group). (c) TAMs were isolated using F4/80⁺ magnetic bead selection and cultured in complete DMEM for 24 hours. Culture supernatants were analyzed by ELISA for murine VEGF, IL-10, and TNF-α (n = 3 per group). *p < 0.05, **p < 0.01, ***p < 0.005 vs. vehicle; ns = nonsignificant. Cytokine data are representative of 3 biologically independent experiments.