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. 2020 Apr 16;10:6519. doi: 10.1038/s41598-020-63561-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Diversity rarefaction curve of the V4 (dotted line) and V9 (solid line) operational taxonomic units (OTUs) with 97% identity threshold. The saturation of curve indicates that the diversity of OTUs in the present study was mostly covered by the V4 and V9 sequences.