Fig. 4.

WWOX modulates TGF-β signaling via miR-146a targeting of SMAD3. a Immunoblot of SMAD3 in MDA-MB435 cells; Ev-empty vector, WFPA-expressing mutated WWOX, WWOX-expressing wild-type WWOX. b Luciferase assay of SMAD3 3′UTR (wild-wild type, mut-mutated 3′UTR), with or without miR-146a. Bars indicates SD. c Immunoblot showing SMAD3 and SMAD2 levels in MDA-MB435S cells upon expression of miR-146a or miR-363. d Immunoblot showing Smad3 in MDA-MB435S cells; Ev-empty vector, and WWOX-expressing wild-type WWOX, and assays were performed after expression of anti-miR-146a or anti-Scr for 24 and 48 h. GAPDH was used as an endogenous control in (a), (c) and (d). Densitometry analysis for SMAD3 levels is shown below each blot