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. 2020 Apr 16;10:6499. doi: 10.1038/s41598-020-63403-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ccRCC cell line MZ2733RC as a model for the identification of unknown CREB1-specific post-transcriptional regulators. (A) Western blot-based detection and densitometric quantification of CREB1 protein in the ccRCC cell line MZ2733RC and the corresponding normal tissue cell line MZ2733NN. Number in parentheses indicates a technical replicate. Same blot was re-probed for β-Actin which served as loading control. Uncropped blot is shown in Supplementary Figure 3. (B) CREB1 mRNA levels analyzed by qRT-PCR in the indicated cell lines. GAPDH was used for normalization. (C) Expression levels of validated CREB1-specific miRNAs given as fold enrichment for MZ2733RC (RCC) cell line relative to MZ2733NN (NN) cell line analyzed by RNA-sequencing and qRT-PCR. Values above 1 (red line) indicate miRNAs enriched in the tumor cell line. n.d. not detectable.