Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 16;10:6499. doi: 10.1038/s41598-020-63403-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Validation of CREB1-regulating miRNA candidates. (A) Effect of miRNA mimic or mock transfection on respective miRNA expression in HEK293T cells (left). Western blot-based detection and densitometric quantification of CREB1 after the miRNA mimic or mock transfection in HEK293T cells was performed as described in Materials and Methods. Same blot was re-probed for β-Actin which served as loading control (right). Uncropped blots are shown in Supplementary Figure 5. (B) Illustration of the predicted candidate miRNA binding sites in the 3′-UTR of CREB1 and the mutated versions later used for luc reporter gene assay. (C) Relative luc activity measured after transfection of indicated miRNA mimics or mock control using wt CREB1 3′-UTR or binding site (BS) mutated versions (n = 4, student t-test, *p < 0.05). (D) Analysis of indicated candidate miRNA expression levels given as fold enrichment for MZ2733RC (RCC) cell line relative to MZ2733NN (NN) cell line analyzed by RNA-sequencing and qRT-PCR. Values above 1 (red dotted line) indicate miRNAs enriched in the tumor cell line.