Table 2.

Comparison detection limits of fish nodavirus detection methodologies, i.e. ELISA and lateral flow assays.

	Sensitivity		Reference
	Cultured virus	Infected tissue
Capture/sandwich ELISA	6.5 × 104 TCID50		42
	2–3 × 103 TCID50		43
	35 × 105 TCID50/ mL		44
mAb/LFIA	105.05 TCID50/100 μL		40
Virion LFB	6 × 10–3 TCID50 / 0.5 μL		Present study
RT-PCR/ LFB		135 pg total RNA	8
RT-LAMP–LFD		2 pg total RNA	45
CPA-LFD		101 copies/μL	46