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. 2020 Apr 16;10:6567. doi: 10.1038/s41598-020-63621-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aggregation with different molecular weights of polysaccharides in MC medium. (a) Three different molecular weights of FITC-dextran with average sizes of 10,000, 40,000 and 250,000 were injected into MC medium. The time-dependent dextran distribution was observed (a), and fluorescent areas were quantified (b). Bar = 200 µm. The data are the means ± SEMs, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA vs. the 10,000 group at each time point. (c) Hep G2 cells labelled with PKH26 (red) and molecular weight of 250 FITC-dextran (green) were mixed and injected into MC medium. After one day of culture, aggregation was observed. Bar = 200 µm.