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. 2020 Apr 16;10:6551. doi: 10.1038/s41598-020-63055-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-qPCR results using size-dependent enrichment (Parsortix) and EpCAM-dependent CTC enrichment for: (A) Β2Μ (reference gene); (B) CD-45 (specific leukocyte marker) (C) Relative fold change values (2^-ΔΔCq) for reference gene (Β2Μ) and target gene (PD-L1) for healthy individuals and HNSCC patients’ CTC samples; (D) Relative fold change values (2^-ΔΔCq) for reference gene (Β2Μ) and target gene (TWIST1); (E) Relative fold change values (2^-ΔΔCq) for reference gene (Β2Μ) and target gene (CDH2) Red horizontal line: cutoff value. Different colors represent individual patients.