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. 2020 Apr 16;11(4):233. doi: 10.1038/s41419-020-2426-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a According to KEGG pathway analysis by DAVID6.8, 39 miR-3619-5p target genes related to AMPK pathway were sort out. RT-qPCR results of 39 gene levels under miR-3619-5p overexpression versus control in GC cells. b Western blot results of the levels of p-AMPK and PGC1α in GC cells with or without MSC co-culture. c MiR-3619-5p, PPARGC1A mRNA, and HCP5 were all enriched in the immunoprecipitated products of Ago2. d MiR-3619-5p sites on PPARGC1A were predicted by starBase3.0, the mutated sites were designed for luciferase reporter assay. Luciferase activity of PPARGC1A WT/Mut was assessed in 293T cells under overexpression of miR-3619-5p versus control. e, f RT-qPCR results of PPARCG1A level and western blot results of PGC1α and CPT1 level in GC cells with indicated transfection. g Luciferase activity of CPT1 promoter reporter in 293T cells under indicated transfection. h RT-qPCR data showed the levels of PPARGC1A mRNA in GC cells of each group. i ChIP assay revealed that overexpression of HCP5 led to increased enrichment of CPT1 promoter in PGC1α precipitates. j CoIP analysis demonstrated that PGC1α protein in CEBPB precipitates was enhanced by enhancing HCP5, with the expression of CEBPB unchanged. **P < 0.01. Error bars indicate SD. Each assay was conducted for three times.